Anti-GPR49 / LGR5 Antibody (aa689-719)
| Name | Anti-GPR49 / LGR5 Antibody (aa689-719) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C98619 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | ICC/IF WB FC |
| Species Reactivities | Human |
| Purity/Format | Protein A purified |
| Blocking Peptide | GPR49 / LGR5 Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | LGR5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent confocal image of HeLa cells stained with LGR5 (loop2) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000139292).
Western blot of anti-LGR5/GPR49 Antibody (loop2) in mouse cerebellum tissue lysates (35 ug/lane). LGR5/GPR49(arrow) was detected using the purified antibody.
LGR5/GPR49 Antibody (loop2) western blot of HepG2 cell line lysates (35 ug/lane). The LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).
Flow cytometric of HepG2 cells using LGR5/GPR49 Antibody (loop2) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.