Western blot analysis of extracts from various cell lines using IRF-5 (E1N9G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of THP-1 (left) and K-562 (right) cells using IRF-5 (E1N9G) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of THP-1 cells using IRF-5 (E1N9G) Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.