Western blot analysis of extracts from Karpas-620 (human) and A20 (mouse) cell lines using IRF-4 Antibody #4964 and IRF-4 (P173) Antibody #4948.
Western blot analysis of extracts from Ramos, Raji, and HuT-78 cells, using IRF-4 Antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 H929 cells and either 20 μl of IRF-4 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human SUB1 Promoter Primers #5156 and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.