Anti-HAUS8 Antibody IHC-plus™

• Supplier: LifeSpan Bioscience
• Catalog: LS-B10617
• Prices: $395.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA
• Species Reactivities: Human
• Antigen: HAUS8 antibody was raised against anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1.
• Purity/Format: Immunoaffinity purified
• Description: Rabbit Polyclonal
• Gene: HAUS8
• Conjugate: Unconjugated

Product images

Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10617 concentration 5 ug/ml.

Western Blot of Rabbit anti-HAUS8 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 µl per lane. Primary antibody: HAUS8 antibody at 1.24 ug/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HAUS8. Other band(s): HAUS8 splice variants and isoforms.

Western blot of Rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1. Other band(s): none.