HAUS8

Gene Symbol HAUS8
Entrez Gene 93323
Alt Symbol DGT4, HICE1, NY-SAR-48
Species Human
Gene Type protein-coding
Description HAUS augmin-like complex, subunit 8
Other Description HAUS augmin-like complex subunit 8|HEC1/NDC80 interacting, centrosome associated 1|HEC1/NDC80-interacting centrosome-associated protein 1|Hec1-interacting and centrosome-associated 1|sarcoma antigen NY-SAR-48
Swissprots Q9BT25 Q86WF0 C9JBZ4 B4DJA7 Q49AC4 Q96FX3
Accessions EAW84570 EAW84571 Q9BT25 AK295994 BAG58769 AY211919 AAO65172 BC004398 AAH04398 BC010176 AAH10176 BC040564 BI829542 BM563712 BX111116 XM_005260154 XP_005260211 XM_011528438 XP_011526740 NM_001011699 NP_001011699 NM_033417 NP_219485
Function Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. {ECO:0000269|PubMed:18362163, ECO:0000269|PubMed:19369198, ECO:0000269|PubMed:19427217}.
Subcellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, additionally associates with the spindle midzone and midbody, respectively.

HICE1 (E-14) - sc-240745 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HICE1 (E-14): sc-240745. Western blot analysis of HICE1 expression in HEK293 whole cell lysate.

HICE1 (Q-14) - sc-240746 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HICE1 (Q-14): sc-240746. Western blot analysis of HICE1 expression in HEK293 whole cell lysate.

Anti-NYS48 antibody - ab95970 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NYS48 antibody (ab95970) at 1/500 dilutionLane 1 : His-Hice 1 (2x)Lane 2 : His-Hice 1 (1x)

Anti-NYS48 antibody - ab95459 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NYS48 antibody - ab67052 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Anti-NYS48 antibody (ab67052) at 1/500 dilution + human spleen tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-NYS48 antibody - ab88968 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-NYS48 antibody - ab181633 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NYS48 antibody (ab181633) at 1/10000 dilutionLane 1 : sh-Luciferase lentivirus infected HeLa lysate (control)Lane 2 : sh-NYS48 RNAi-treated lentivirus infected HeLa lysatedeveloped using the ECL technique

Anti-NYS48 antibody - N-terminal - ab173869 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NYS48 antibody - N-terminal (ab173869) at 1/100 dilution + A549 cell line lysate at 35 µg

Anti-HAUS8 Antibody IHC-plus™ - LS-B10617 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10617 concentration 5 ug/ml.

Anti-HAUS8 Antibody (aa166-360) - LS-C185381 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Human lymph tissue using HAUS8 antibody at 1:50 dilution.

Anti-HAUS8 Antibody (aa28-54, AP) - LS-C236109 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HAUS8 Antibody (aa28-54, APC) - LS-C236110 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HAUS8 Antibody (aa28-54, Biotin) - LS-C236111 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HAUS8 Antibody (aa28-54, FITC) - LS-C236112 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HAUS8 Antibody - LS-C153765 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-HICE1 in Western blot of Immunochemicals' Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

Anti-HAUS8 Antibody (aa28-54, PE) - LS-C236113 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HAUS8 Antibody (aa28-54, Azide-free, HRP) - LS-C249164 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HAUS8 Antibody (aa57-106) - LS-C135653 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HAUS8 antibody LS-C135653 Western blot of HeLa lysate.

Anti-HAUS8 Antibody - LS-C308182 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-HAUS8 Antibody (phospho-Ser70) - LS-C153858 from LifeSpan Bioscience

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 pS70 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1 pS70. Other band(s): none.

Anti-HAUS8 Antibody (aa27-54) - LS-C321879 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HAUS8 Antibody (aa28-54) - LS-C159810 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
HAUS8 Antibody western blot of A549 cell line lysates (35 ug/lane). The HAUS8 antibody detected the HAUS8 protein (arrow).

NYS48 Antibody - NBP1-79780 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NYS48 Antibody [NBP1-79780] - Hela cell lysate, concentration 0.125ug/ml.

NYS48 Antibody - H00093323-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: NYS48 Antibody [H00093323-B01] - NY-SAR-48 MaxPab polyclonal antibody (B01), Lot # 07285WULz. Western Blot analysis of NY-SAR-48 expression in human spleen.

NYS48 Antibody - H00093323-B01P from Novus Biologicals

Prices $349.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: NYS48 Antibody [H00093323-B01P] - NY-SAR-48 MaxPab polyclonal antibody. Western Blot analysis of NY-SAR-48 expression in human spleen.
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