Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection of Human USP7 by Western Blot. Samples: Nuclear extract (50 ug/lane) from HeLa cells. Antibody: Affinity purified rabbit anti-USP7 antibody used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
Detection of Human USP7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and K562 cells. Antibodies: Affinity purified rabbit anti-USP7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat USP7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-USP7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.