Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent of HDGF Antibody with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot of anti-HDGF antibody in HeLa cell line lysates (35 ug/lane). HDGF(arrow) was detected using the purified antibody.