HDGF

Gene Symbol HDGF
Entrez Gene 3068
Alt Symbol HMG1L2
Species Human
Gene Type protein-coding
Description hepatoma-derived growth factor
Other Description HMG-1L2|high mobility group protein 1-like 2
Swissprots D3DVC9 P51858 B3KU21 Q5SZ09 Q5SZ07 Q5SZ08
Accessions EAW52910 EAW52911 EAW52912 P51858 AK096411 BAG53283 AK292325 BAF85014 AK298342 BAH12761 AK304468 BAH14194 AK315514 BAG37895 AL833592 AU131311 BC018991 AAH18991 CN392158 CN396181 D16431 BAA03903 DA207052 DA367806 DQ893406 ABM84332 DQ896724 ABM87723 DR159090 F36780 XM_006711280 XP_006711343 XM_006711281 XP_006711344 XM_011509453 XP_011507755 XM_011509454 XP_011507756 XM_011509455 XP_011507757 XM_011509456 XP_011507758 NM_001126050 NP_001119522 NM_001126051 NP_001119523 NM_004494 NP_004485
Function Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. {ECO:0000269|PubMed:17974029}.
Subcellular Location Cytoplasm. Nucleus.
Tissue Specificity Ubiquitous.

HDGF (E-7) - sc-271344 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDGF (E-7): sc-271344. Western blot analysis of HDGF expression in EOC 20 (A) and KNRK (B) whole cell lysates.

HDGF (H-3) - sc-398344 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDGF (H-3): sc-398344. Western blot analysis of HDGF expression in A549 (A) and MCF7 (B) whole cell lysates.

HDGF (C-14)-R - sc-28064-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
HDGF (C-14): sc-28064. Western blot analysis of HDGF expression in ECV304 (A) and HeLa (B) whole cell lysates.

HDGF (N-15) - sc-28062 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HDGF (N-15): sc-28062. Western blot analysis of HDGF expression in ECV304 (A) and A549 (B) whole cell lysates.

HDGF (H-48) - sc-292798 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HDGF (H-48): sc-292798. Western blot analysis of HDGF expression in non-transfected 293T: sc-117752 (A), mouse HDGF transfected 293T: sc-120729 (B), A549 (C) and EOC 20 (D) whole cell lysates.

Anti-HDGF antibody - ab196574 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling HDGF with ab196574 at a 1/200 dilution.

Anti-HDGF antibody - ab176697 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDGF antibody (ab176697) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : Jurkat whole cell lysate at 50 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg

Anti-HDGF antibody - ab135569 from Abcam

Prices $304.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-HDGF antibody (ab135569) at 1/50 dilution + HeLa cell line lysates at 35 µg

Anti-HDGF antibody - ab113209 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of HDGF by Western Blot of Immunprecipitate. An antibody to HDGF at 1µg/ml staining HDGF in HeLa whole cell lysate immunoprecipitated using ab113209 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 second.

Anti-HDGF antibody [26C4a] - ab58644 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 26C4a
Anti-HDGF antibody [26C4a] (ab58644) at 1/100 dilution + immunising recombinant protein at 25 µgSecondarySheep Anti Mouse IgG at 1/3000 dilution

Anti-HDGF antibody [EPR7898] - ab128921 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7898
Overlay histogram showing HeLa cells stained with ab128921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-HDGF antibody [EPR7898] (Alexa Fluor® 488) - ab201766 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7898
ab201766 staining HDGF in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201766 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Anti-HDGF antibody [EPR7898] (Alexa Fluor® 647) - ab201270 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7898
ab201270 staining HDGF in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201270 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-HDGF antibody [EPR7899] - ab131046 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7899
All lanes : Anti-HDGF antibody [EPR7899] (ab131046) at 1/10000 dilutionLane 1 : A549 cell lysateLane 2 : Saos-2 cell lysateLane 3 : ECV-304 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HDGF Antibody (C-Terminus, clone EPR7899) - LS-C154517 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7899
IHC of paraffin-embedded human colon tissue using anti-HDGF antibody.

Anti-HDGF Antibody (clone 2D6) - LS-C197402 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D6
Immunofluorescence of monoclonal antibody to HDGF on HeLa cell . [antibody concentration 10 ug/ml]

Anti-HDGF Antibody (Internal, clone EPR7898) - LS-C152555 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7898
IHC of paraffin-embedded human colon tissue using anti-HDGF antibody.

Anti-HDGF Antibody (aa171-197) - LS-C98707 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-HDGF Antibody (clone 26C4a) - LS-C342596 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 26C4a
Western blot analysis of immunized recombinant protein, using anti-HDGF monoclonal antibody.

Anti-HDGF Antibody - LS-C333997 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human stomach using HDGF antibody at dilution of 1:200 (400x lens).

Anti-HDGF Antibody - LS-C67685 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-HDGF Antibody (clone 26C4a) - LS-C184558 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 26C4a

Anti-HDGF Antibody (aa100-150) - LS-C289868 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HDGF Antibody (aa150-200) - LS-C289869 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HDGF Antibody (aa200-240) - LS-C289870 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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