
Anti-HIBCH mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.

Western blot of extracts (35 ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.

Flow cytometry of Jurkat cells, using anti-HIBCH antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HIBCH antibody, and then analyzed by flow cytometry.