Western blot analysis of extracts from various cell lines using JunB (C37F9) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using JunB (C37F9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Confocal immunofluorescent analysis of MCF7 cells using JunB (C37F9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of JunB (C37F9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP ® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP ® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.