JUNB

Gene Symbol JUNB
Entrez Gene 3726
Alt Symbol AP-1
Species Human
Gene Type protein-coding
Description jun B proto-oncogene
Other Description activator protein 1|transcription factor jun-B
Swissprots P17275 Q96GH3
Accessions AAA59198 AAA74915 AAU43800 EAW84307 P17275 AK222532 BAD96252 AK313737 BAG36478 BC004250 AAH04250 BC009465 AAH09465 BC009466 AAH09466 BC110372 BT019760 AAV38565 DQ650707 DQ893021 ABM83947 DQ896266 ABM87265 X51345 CAA35738 NM_002229 NP_002220
Function Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Subcellular Location Nucleus.
Top Pathways Osteoclast differentiation, TNF signaling pathway

Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb - 8053 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3C6
Western blot analysis of extracts from 293T cells treated with Anisomycin (25 ug/ml) for the time indicated, with or without pretreatment with JNK inhibitor SP600125 (40 min, 50 uM), using Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb (upper) or JunB (C37F9) Rabbit mAb #3753 (lower).

JunB (C37F9) Rabbit mAb - 3753 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C37F9
Western blot analysis of extracts from various cell lines using JunB (C37F9) Rabbit mAb.

JunB (G53) Antibody - 3746 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of total cell lysates from HeLa, MCF-7 and SW620 cells using JunB (G53) Antibody.

JunB (P169) Antibody - 3755 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of total cell lysates from various cell lines using JunB (P169) Antibody.

Jun B (210) - sc-73 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Jun B (210): sc-73. Western blot analysis of Jun B expression in phorbol ester-induced NIH/3T3 (A), untreated KNRK (B) and phorbol ester-induced KNRK (C) nuclear extracts.

Jun B (G-9) - sc-398061 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Jun B (G-9): sc-398061. Western blot analysis of Jun B expression in HeLa (A), NIH/3T3 (C) and KNRK (D) nuclear extracts and Caki-1 whole cell lysate (B). Note lack of reactivity in lanes C and D.

Jun B (C-11) - sc-8051 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Jun B (C-11): sc-8051. Western blot analysis of Jun B expression in KNRK nuclear extract from PMA-treated cells.

Jun B (N-17) - sc-46 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Jun B (N-17): sc-46. Western blot analysis of Jun B in nuclear extract of phorbol ester-induced KNRK rat cells.

Jun B (H-44) - sc-292490 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-Jun B (Ser 259) - sc-101724 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated HSF1 expression in MCF7 whole cell lysates (A,B). Blots were probed with p-HSF1 (Ser 303): sc-101698 preincubated with its cognate phosphorylated peptide (A) and p-HSF1 (Ser 303): sc-101698 (B).

p-Jun B (Ser 79) - sc-101725 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Jun B (Ser 79): sc-101725. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.

Anti-JunB (phospho S259) antibody - ab30628 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-JunB (phospho S259) antibody (ab30628) at 1/500 dilutionLane 1 : extracts from HeLa cells (5-30ug).Lane 2 : extracts from HeLa cells (5-30ug), preincubated with synthesized non phosphopeptide.Lane 3 : extracts from HeLa cells (5-30ug), preincubated with synthesized phosphopeptide (negative control).

Anti-JunB (phospho S79) antibody - ab30625 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 JunB (Phospho Ser79) Antibody (ab30625). Left: Untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).

Anti-JunB antibody - ab31421 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab31421 stained Hek293 cells (ab7902). The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31421, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-JunB antibody - ab28838 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded breast carcinoma using 1/50 JunB (Ab-1) Antibody (ab28838). Left: Untreated sample Right: Sample preincubated with synthesized peptide (negative control).

Anti-JunB antibody - ab90565 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-JunB antibody (ab90565) at 1 µg/ml + Human liver tissue lysate at 50 µg

Anti-JunB antibody [204C4a] - ab53543 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 204C4a
Anti-JunB antibody [204C4a] (ab53543) + Immunized recombinant protein

Anti-JunB antibody [EPR6518] - ab128878 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6518
All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilutionLane 1 : MCF-7 lysateLane 2 : Human tonsil lysateLane 3 : Human uterus cancer lysateLane 4 : HeLa lysateLane 5 : HACAT lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-JunB antibody [EPR6518] (Alexa Fluor® 488) - ab200515 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6518
ab200515 staining JunB in MCF7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200515 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-JunB antibody [EPR6518] (Alexa Fluor® 647) - ab200516 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6518
ab200516 staining JunB in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200516 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-JUNB / JUN-B Antibody (Internal) - LS-C24929 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-JUNB / JUN-B Antibody (N-Terminus) - LS-C30825 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate

Anti-JUNB / JUN-B Antibody (aa271-320) - LS-C30353 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate

Anti-JUNB / JUN-B Antibody - LS-C71176 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-JUNB / JUN-B Antibody (aa241-290) - LS-C30824 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate
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