Fluorescent confocal image of HeLa cell stained with HNRNPD Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRNPD primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HNRNPD immunoreactivity is localized to Nucleus and Cytoplasm significantly.
HNRNPD Antibody western blot of 293T,A431,NCI-H292,Raji cell line lysates (35 ug/lane). The HNRNPD antibody detected the HNRNPD protein (arrow).