Western blot analysis of extracts from various cell lines using Keratin 17 (D12E5) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of HeLa (positive, left) and HCC827 (negative, right) cells using Keratin 17 (D12E5) XP ® Rabbit mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of SK-MEL-28 (blue) and HeLa (green) cells using Keratin 17 (D12E5) XP ® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) #4414 was used as a secondary antibody.