Detection of Human Lasu1/Ureb1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human Ureb1/Lasu1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Ureb1/Lasu1 antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Ureb1/Lasu1 , was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Mouse Ureb1/Lasu1 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Ureb1/Lasu1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human Ureb1/Lasu1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), and HeLa (50 ug) cells. Antibodies: Affinity purified rabbit anti-Ureb1/Lasu1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.