HUWE1

Gene Symbol HUWE1
Entrez Gene 10075
Alt Symbol ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, URE-B1, UREB1
Species Human
Gene Type protein-coding
Description HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
Other Description ARF-binding protein 1|BJ-HCC-24 tumor antigen|E3 ubiquitin-protein ligase HUWE1|HECT domain protein LASU1|Mcl-1 ubiquitin ligase E3|URE-binding protein 1|homologous to E6AP carboxyl terminus homologous protein 9|large structure of UREB1|upstream regulatory element-binding protein 1
Swissprots Q9NSL6 Q9HCJ4 Q6P4D0 Q4G2Z2 Q9P0A9 Q7Z6Z7 O15029 Q5H961 Q8NG67 Q9BUI0
Accessions CAI39578 CAI39579 CAI39580 CAI39581 CAI42351 CAI42352 CAI42353 CAI42354 CAI42644 CAI42654 CAI42656 CAJ47378 CCQ43744 EAW93160 EAW93161 EAW93162 EAW93163 EAW93164 EAW93165 EAW93166 Q7Z6Z7 AB002310 BAA20771 AB071605 BAC06833 AF057569 AAC62492 AF161390 AAF28950 AK056671 AK091352 AK223167 BAD96887 AK294575 BAG57767 AL162050 CAB82393 AY772009 AAV90838 AY929612 AAX24125 BC002602 AAH02602 BC018750 AAH18750 BC038184 AAH38184 BC063505 AAH63505 BC072421 BC107576 AAI07577 BG334536 BU619074 CK002817 CR456813 CAG33094 DQ097177 AAY98258 R60532 XM_005261965 XP_005262022 XM_011530746 XP_011529048 XM_011530747 XP_011529049 XM_011530748 XP_011529050 XM_011530749 XP_011529051 XM_011530750 XP_011529052 XM_011530751 XP_011529053 XM_011530752 XP_011529054 XM_011530753 XP_011529055 XM_011530754 XP_011529056 XM_011530755 XP_011529057 XM_011530756 XP_011529058 XM_011530757 XP_011529059 XM_011530758 XP_011529060 XR_938360 NM_031407 NP_113584
Function E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4. {ECO:0000269|PubMed:15567145, ECO:0000269|PubMed:15767685, ECO:0000269|PubMed:15989956, ECO:0000269|PubMed:15989957, ECO:0000269|PubMed:17567951, ECO:0000269|PubMed:18488021, ECO:0000269|PubMed:19037095, ECO:000026
Subcellular Location Cytoplasm {ECO:0000269|PubMed:19713937}. Nucleus {ECO:0000269|PubMed:19713937}. Note=Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells. {ECO:0000250|UniProtKB:Q7TMY8}.
Tissue Specificity Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas. {ECO:0000269|PubMed:15567145}.
Top Pathways Ubiquitin mediated proteolysis

HECTH9 (AX8D1) Mouse mAb - 5695 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone AX8D1
Western blot analysis of extracts from various cell lines using HECTH9 (AX8D1) Mouse mAb.

URE-B1 (H-300) - sc-134821 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

URE-B1 (Y-19) - sc-49768 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
URE-B1 (Y-19): sc-49768. Western blot analysis of URE-B1 expression in HCT-116 (A) and U-2 OS (B) whole cell lysates.

URE-B1 (N-17) - sc-49767 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-ARFBP1 antibody - ab70161 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling ARFBP1 with ab70161 at 1/1000 (1µg/ml). Detection: DAB.

Anti-ARFBP1 antibody - ab65153 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab65153 at 5µg/ml staining ARFBP1 in Brain, cortex tissue.

Anti-ARFBP1 antibody [AX81] - ab78397 from Abcam

Prices $359.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone AX81
Immunofluorescence Analysis of ARFBP1 in HeLa Cells using ab78397 at 1/200 dilution.

Anti-HUWE1 / ARFBP1 Antibody (aa1025-1075) - LS-C93263 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HUWE1 / ARFBP1 Antibody (aa4251-4263) - LS-C112841 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-HUWE1 / ARFBP1 Antibody (aa2530-2596) - LS-C152880 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
FMAT Analysis: HeLa Cells Stained with a 1/200 dilution of anti-Hecth9 ascites

Anti-HUWE1 / ARFBP1 Antibody (aa2250-2300) - LS-C286667 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Lasu1/Ureb1 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 used at a dilution of 1:250.

Anti-HUWE1 / ARFBP1 Antibody (aa2250-2300) - LS-C290483 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
Detection of Human Lasu1/Ureb1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified goat anti-Lasu1/Ureb1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-HUWE1 / ARFBP1 Antibody - LS-C343774 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of full length Huwe1 (~482kDa) and its degradation fragments in mouse testis lysate (50ug) by Western blotting using PW0950 at 1:100 dilution.

Anti-HUWE1 / ARFBP1 Antibody (aa2250-2300) - LS-C287372 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Lasu1/Ureb1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-HUWE1 / ARFBP1 Antibody (C-Terminus) IHC-plus™ - LS-B1359 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-HUWE1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1359 concentration 5 ug/ml.

HUWE1 Antibody - NB100-652 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: HUWE1 Antibody [NB100-652] - Sample: FFPE section of human ovarian carcinoma.~~Antibody: Affinity purified rabbit anti-Lasu1/Ureb1~ used at a dilution of 1:1,000 (1ug/ml).~ Detection: DAB Counterstain: IHC Hematoxylin (blue).

HUWE1 Antibody - NBP1-76795 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

HUWE1 Antibody - NBP1-83127 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-83127] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

HUWE1 Antibody - NBP1-45233 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

HUWE1 Antibody (AX81) - NBP2-29784 from Novus Biologicals

Prices $469.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AX81

HUWE1 Antibody - H00010075-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

HUWE1--LASU1-Antibody-internal-region - AF3010a from Abgent, a WuXi AppTec company

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

HUWE1 antibody - GTX85051 from GeneTex

Prices $329.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Anti-ARF-BP1 antibody used in ICC/IF. GTX85051

HUWE1 antibody, Internal - GTX88364 from GeneTex

Prices $239.00
Sizes 100 μg
Host Goat
Clonality Polyclonal

HUWE1 antibody [C2C3], C-term - GTX109706 from GeneTex

Prices $149.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal
Anti-HUWE1 antibody [C2C3], C-term used in WB-Ag. GTX109706
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