Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 13448
• Prices: $119.00, $318.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D2B2E
• Applications: WB IP ICC/IF
• Species Reactivities: Human, Mouse, Rat
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser22 of human lamin A/C protein.
• Description: Rabbit Monoclonal
• Gene: LMNA

Product images

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, treated with either hydroxyurea (4 mM, 20 hr; +) to induce G1/S phase or Paclitaxel #9807 (100 nM, 20 hr; +) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb (upper) and Lamin A/C Antibody #2032 (lower).

Immunoprecipitation of phospho-Lamin A/C (Ser22) from extracts of HeLa cells treated with Paclitaxel #9807, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Lamin A/C (Ser22) (D2B2E) X ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or hydroxyurea-treated (right), using Phospho-Lamin A/C (Ser22) (D2B2E) XP ® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).