Anti-Human IL-29 Antibody - Western Blot. Anti-human IL-29 antibody in western blot shows detection of recombinant human IL-29 raised in E. coli. Recombinant protein (0.1 ug, 19.9 kD) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Human IL-29. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.