Western blot analysis of extracts from COS cells, transfected with 5-LO wild type or GFP 5-LO (Ser663Ala), using Phospho-5-Lipoxygenase (Ser663) Antibody. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide (left), with 5-LO (Ser663) non-phosphopeptide (middle) or 5-LO (Ser663) phosphopeptide (right) prior to incubating the membrane.
Western blot analysis of extracts from COS cells, untransfected, or transfected with either 5-LO wild type or GFP 5-LO (Ser663Ala), using Phospho-5-Lipoxygenase (Ser663) Antibody (upper) and 5-Lipoxygenase (C49G1) Rabbit mAb #3289 (lower). (We are thankful to Dr. Flamand from University of Michigan for providing the overexpression constructs and for his help in characterizing this antibody).