Anti-ING4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B386 concentration 5 ug/ml.
![Anti-p29 ING4 Polyclonal Antibody - Western Blot. Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot. This antibody was used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at ~29 kD corresponding to ING4. A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] (code for 45 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_3/15063_62763_49229.jpg)
Anti-p29 ING4 Polyclonal Antibody - Western Blot. Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot. This antibody was used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at ~29 kD corresponding to ING4. A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] (code for 45 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-p29 ING4 Polyclonal Antibody - Western Blot. Anti-p29 ING4 polyclonal antibody detects ING4 protein by Western blot of over expressed cell lysates. This antibody was used at 1.0 ug/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells. Personnel Communication, Motoko Unoki, NCI, NIH.