Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-InsRR antibody in A375 cell lysate. Lane A: preimmune, Lane B: purified antibody. InsRR (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.