Western blot analysis of gp130 expression in HeLa (A,C) and WEHI-231 (B,D) whole cell lysates. Antibodies tested include gp130 (C-20): sc-655 (A,B) and gp130 (M-20): sc-656 (C,D).
gp130 (M-20): sc-656. Western blot analysis of gp130 expression in c4 whole cell lysate.
Western blot analysis of gp130 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) c4 whole cell lysates. Antibodies tested include p-gp130 (Ser 782)-R: sc-12978-R (A,B) and gp130 (M-20): sc-656 (C,D).
gp130 (M-20): sc-656. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.