Western blot analysis of HeLa cell extracts, untreated (-) or treated with Nocodazole #2190 (100 ng/ml, 24 hr; +), using Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb (upper), MCM3 (D47B6) Rabbit mAb #4003 (middle) and α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).
Immunoprecipitation of Phospho-MCM3 (Ser112) from HeLa cell extracts, untreated (-) or treated with Nocodazole #2190 (100 ng/ml, 24 hr; +), using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 3 and 4) or Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb (lane 5 and 6). Lanes 1 and 2 are 10% input. Western blot analysis was performed using Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb.