Immunofluorescence of anti-KITLG Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Western blot of KITLG(arrow) using rabbit polyclonal KITLG Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).
Western blot of KITLG Antibody in 293 cell line lysates (35 ug/lane). KITLG (arrow) was detected using the purified antibody.
Flow cytometric of WiDr cells using SCF (KITLG) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.