Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® MEK2 siRNA II (+), using MEK2 (13E3) Rabbit mAb #9147 and α-Tubulin (11H10) Rabbit mAb #2125. MEK2 (13E3) Rabbit mAb confirms silencing of MEK2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of MEK2 siRNA.
Western blot analysis of extracts from 293, L929, PC12 and COS cells, using MEK2 (13E3) Rabbit mAb.