Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 13281
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D5A4I
• Applications: WB IP
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser518 of human merlin protein.
• Description: Rabbit Monoclonal
• Gene: NF2

Product images

Western blot analysis of extracts from PC-3 cells and wild-type (wt) and Nf2 knockout (-/-) mouse embryonic fibroblasts (MEFs), untreated (-) or λ phosphatase-treated (+), using Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb (upper), Merlin (D3S3W) Rabbit mAb #12888 (middle), or Ezrin/Radixin/Moesin Antibody #3142 (lower). (MEF wt and MEF Nf2 (-/-) cells were kindly provided by Dr. Andrea McClatchey, MGH Cancer Center and Harvard Medical School, Charlestown MA).

Immunoprecipitation of Phospho-Merlin (Ser518) protein from PC-3 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb.