Hormone-sensitive Lipase/HSL in Human Testis. Hormone-sensitive Lipase/HSL was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody at 8 ug/ml overnight at 4°C. Tissue was stained using Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of spermatocytes.
Detectionof Human and Mouse Hormone-sensitive Lipase/HSL by Western Blot. Western blot shows lysates of MCF7 human breast cancer cell line, NTera2 human testicular embryonic carcinoma cell line, SVEC410 mouse vascular endothelial cell line, and HT29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific bands were detected for Hormone-sensitive Lipase/HSL at approximately 70 kD and 130 kD (as indicated). This experiment was conducted under reducing conditions.