Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb
| Name | Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 12280 |
| Prices | $287.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D8E9 |
| Applications | WB IP ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Monkey, Zebrafish, Bovine, Guinea Pig |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser189 of human MKK3 protein and Ser207 of human MKK6 protein. |
| Description | Rabbit Monoclonal |
| Gene | MAP2K3 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb (upper), MKK3 (D4C3) Rabbit mAb #8535 (middle), or MKK6 (D31D1) Rabbit mAb #8550 (lower).
Immunoprecipitation of phospho-MKK3 (Ser189)/MKK6 (Ser207) from HeLa cells, untreated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb (lanes 3 and 4) or Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lanes 5 and 6). Lanes 1 and 2 are 10% input. Western blot analysis was performed using
Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.