Anti-MAP1LC3B / LC3B Antibody (aa1-30)
| Name | Anti-MAP1LC3B / LC3B Antibody (aa1-30) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C165711 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC IHC-P WB IP |
| Species Reactivities | Human, Rat, Zebrafish |
| Purity/Format | Protein A purified |
| Blocking Peptide | MAP1LC3B / LC3B Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | MAP1LC3B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Immunofluorescent of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Immunofluorescent of U251 cells, using LC3 Antibody (APG8B). U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Western blot of anti-LC3 (APG8b) antibody in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2 ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified antibody.
Western blot of anti-LC3 (APG8b) antibody in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
Western blot of anti-LC3 (APG8b) in untreated or treated HeLa cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
Western blot of lysates from NIH/3T3 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (upper) or Beta-actin (lower).
Western blot of lysates from HepG2 cell line, untreated or treated with chloroquine, using LC3 Antibody (upper) or Beta-actin (lower).
Western blot of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (upper) or Beta-actin (lower).
Western blot of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Product References
Active ras triggers death in glioblastoma cells through hyperstimulation of - Active ras triggers death in glioblastoma cells through hyperstimulation of
Overmeyer JH, Kaul A, Johnson EE, Maltese WA. Mol Cancer Res. 2008 Jun;6(6):965-77.
Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C - Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C
Su WC, Chao TC, Huang YL, Weng SC, Jeng KS, Lai MM. J Virol. 2011 Oct;85(20):10561-71.
Caspase-6 activity in a BACHD mouse modulates steady-state levels of mutant - Caspase-6 activity in a BACHD mouse modulates steady-state levels of mutant
Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimaki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. J Neurosci. 2012 May 30;32(22):7454-65.