Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of lysates from 293, T47D cell line (from left to right), using APG8c (MAP1LC3C) Antibody (M1). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
The anti-LC3 (APG8c) antibody is used in Western blot to detect LC3 (APG8c) in, from left to right, 293, Ramos, HeLa, HepG2, T47d, and Jurkat tissue lysates. LC3 (APG8c)(arrow) was detected using the purified antibody.