Western blot analysis of PDGFR-β phosphorylation in untreated (A,D), PDGFββ treated (B,E) and PDGFββ and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-PDGFR-β (F-10): sc-365464 (A,B,C) and PDGFR-β (11H4): sc-80991 (D,E,F).
Western blot analysis of PDGFR-β phosphorylation in non-transfected: sc-117752 (A,D), untreated human PDGFR-β transfected: sc-159386 (B,E) and lambda protein phosphatase (sc-200312A) treated human PDGFR-β transfected: sc-159386 (C,F) 293T whole cell lysates. Antibodies tested include p-PDGFR-β (F-10): sc-365464 (A,B,C) and PDGFR-β (11H4): sc-80991 (D,E,F).
p-PDGFR-β (F-10): sc-365464. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.