Anti-MAPKAPK5 / PRAK Antibody (aa306-318) IHC-plusâ¢
| Name | Anti-MAPKAPK5 / PRAK Antibody (aa306-318) IHC-plus⢠|
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-B9567 |
| Prices | $395.00 |
| Sizes | 50 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Applications | IHC-P WB ELISA |
| Species Reactivities | Human |
| Antigen | MAPKAPK5 / PRAK antibody was raised against synthetic peptide C-STEALDNVLPSAQ from an internal region of human MAPKAPK5 / PRAK (NP_003659.2; NP_620777.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Ferret, Elephant, Panda, Bovine, Dog, Cat, Horse, Rabbit, Pig, Guinea pig, Lizard (100%); Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Medaka, Pufferfish, Zebrafish (92%). |
| Purity/Format | Immunoaffinity purified |
| Blocking Peptide | MAPKAPK5 / PRAK Antibody Blocking Peptide |
| Description | Goat Polyclonal |
| Gene | MAPKAPK5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-MAPKAPK5 / PRAK antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9567 concentration 5 ug/ml.
MAPKAPK5 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with (0.5 ug/ml) in right panel and with EB11928 (0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.
MAPKAPK5 antibody and EB11928 (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).