MAPKAPK5

Gene Symbol MAPKAPK5
Entrez Gene 8550
Alt Symbol MAPKAP-K5, MK-5, MK5, PRAK
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase-activated protein kinase 5
Other Description MAP kinase-activated protein kinase 5|MAPK-activated protein kinase 5|MAPKAP kinase 5|MAPKAPK-5|p38-regulated/activated protein kinase
Swissprots Q86X46 B3KVA5 Q9UG86 Q9BVX9 O60491 Q8IW41
Accessions AAC39863 EAW97979 EAW97980 EAW97981 Q8IW41 AK122767 BAG53717 AL110301 CAB53747 AW780199 BC000833 AAH00833 BC041049 AAH41049 BC047284 AAH47284 BG502445 XM_005253979 XP_005254036 XM_011538909 XP_011537211 XM_011538910 XP_011537212 XM_011538911 XP_011537213 XM_011538912 XP_011537214 XM_011538913 XP_011537215 XM_011538914 XP_011537216 XM_011538915 XP_011537217 XM_011538916 XP_011537218 XM_011538917 XP_011537219 XM_011538918 XP_011537220 XM_011538919 XP_011537221 XM_011538920 XP_011537222 XM_011538921 XP_011537223 XM_011538922 XP_011537224 XR_944806 XR_944807 NM_003668 NP_003659 NM_139078 NP_620777
Function Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras- induced senescence and phosphorylating p53/TP53. Involved in post- transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ER
Subcellular Location Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.
Tissue Specificity Expressed ubiquitously. {ECO:0000269|PubMed:9628874}.
Top Pathways MAPK signaling pathway

MAPKAPK-5 (D70A10) Rabbit mAb - 7419 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D70A10
Western blot analysis of extracts from various cell lines using MAPKAPK-5 (D70A10) Rabbit mAb.

Phospho-PRAK Antibody - 9771 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

PRAK Antibody - 9772 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

PRAK (A-7) - sc-46667 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PRAK (A-7): sc-46667. Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

PRAK (7H10B4) - sc-81705 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PRAK (7H10B4): sc-81705. Western blot analysis of truncated human recombinant PRAK protein.

PRAK (H-180) - sc-25419 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PRAK (H-180): sc-25419. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

PRAK (K-19) - sc-8253 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PRAK (K-19): sc-8253. Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.

PRAK (N-19) - sc-8251 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PRAK (N-19): sc-8251. Western blot analysis of PRAK expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.

Anti-PRAK (phospho S212) antibody - ab59999 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59999 at 1/50 dilution staining PRAK in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. The primary antibody was peroxidase conjugated to the secondary antibody, followed by AEC staining.

Anti-PRAK (phospho T182) antibody - ab138668 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PRAK (phospho T182) antibody (ab138668) at 1/500 dilution + K562 cell lysate (from cells treated with 0.3 nM sodium orthovanadate for 40 minutes) at 30 µg

Anti-PRAK antibody - ab97332 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRAK antibody (ab97332) at 1/500 dilutionLane 1 : 293T whole cell lysateLane 2 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-PRAK antibody - ab93800 from Abcam

Prices $370.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRAK with ab93800 at 1/200 (1µg/ml). Detection: DAB.

Anti-PRAK antibody - ab79433 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRAK antibody (ab79433) at 1/500 dilutionLane 1 : MCF7 cell extractsLane 2 : MCF7 cell extracts with immunising peptide at 10 µgLysates/proteins at 10 µg per lane.

Anti-PRAK antibody - ab89525 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PRAK antibody (ab89525) at 1 µg/ml + HeLa cells nuclear extracts at 50 µg

Anti-PRAK antibody [7H10B4] - ab66382 from Abcam

Host Mouse
Clonality Monoclonal
Clone 7H10B4
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using ab66382 at a dilution of 1/500 - 1/2000 with DAB staining, showing nuclear/cytoplasmic localization.

Anti-MAPKAPK5 / PRAK Antibody (phospho-Ser93) - LS-C3286 from LifeSpan Bioscience

Prices $285.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MAPKAPK5 / PRAK Antibody (phospho-Thr182) - LS-C17344 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-MAPKAPK5 / PRAK Antibody (Ser93, AP) - LS-C279207 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPKAPK5 / PRAK Antibody - LS-C62049 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-MAPKAPK5 / PRAK Antibody (Ser93, Biotin) - LS-C279208 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPKAPK5 / PRAK Antibody (aa171-220) - LS-C199230 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human brain tissue, using MAPKAPK5 Antibody. The sample on the right was incubated with synthetic peptide.

Anti-MAPKAPK5 / PRAK Antibody (Ser93, APC) - LS-C279209 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPKAPK5 / PRAK Antibody (aa148-197) - LS-C199228 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from MCF-7 cells, using MAPKAPK5 Antibody. The lane on the right was incubated with synthetic peptide.

Anti-MAPKAPK5 / PRAK Antibody (Ser93, FITC) - LS-C279212 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPKAPK5 / PRAK Antibody (Ser93, Azide-free, HRP) - LS-C279213 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more