Western blot analysis of extracts from KARPAS-299, cells untreated (-) or treated with MG-132 #2194 (10 μM, overnight; +) using Phospho-c-Myc (Ser62) (E1J4K) Rabbit mAb. The phospho-specificity of the antibody was demonstrated by peptide competition using phosphopeptides containing Ser62, Thr58/Ser62, Thr58, or a nonphosphorylated peptide as indicated.
Western blot analysis of extracts from KARPAS-299 and HCT 116 cells, untreated (-) or treated with MG-132 #2194 (10 μM, overnight; +), using Phospho-c-Myc (Ser62) (E1J4K) Rabbit mAb (upper), c-Myc (D84C12) XP ® Rabbit mAb #5605 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).