Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with nonspecific (control) siRNA or transfection with c-Myc siRNA. c-Myc was detected using c-Myc Antibody #9402, and p42 was detected using p42 MAPK Antibody #9108. The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity.
Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Daudi cells and either 10 μl of c-Myc Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATF4 promoter primers, SimpleChIP ® Human NPM1 Intron 1 Primers #4779, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.