pan 14-3-3 (H-8): sc-1657. Immunofluorescence stain-ing of methanol-fixed HeLa cells. Note cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain.
pan 14-3-3 (H-8): sc-1657. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Western blot analysis of 14-3-3 expression in HeLa whole cell lysates (A,B). Antibodies tested include pan 14-3-3 (H-8): sc-1657 (A) and pan 14-3-3 (K-19): sc-629 (B).
pan 14-3-3 (H-8) PE: sc-1657 PE. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG
2b: sc-2868.
pan 14-3-3 (H-8) Alexa Fluor 488: sc-1657 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG
2b: sc-3892.
pan 14-3-3 (H-8): sc-1657. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor
® 488 (B) staining.
pan 14-3-3 (H-8): sc-1657. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
pan 14-3-3 (H-8): sc-1657. Western blot analysis of 14-3-3 ζ expression in non-transfected 293: sc-110760 (A), human 14-3-3 ζ transfected 293: sc-175740 (B) and HeLa (C) whole cell lysates.
pan 14-3-3 (H-8): sc-1657. Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic and nuclear staining of neuronal cells.
pan 14-3-3 (H-8): sc-1657. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue. Note cytoplasmic and membrane staining of glandular epithelia.