Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent of MMP12 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot of anti-hMMP12-R406 antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. MMP12(arrow) was detected using the purified antibody;
Western blot of anti-hMMP12-R406 antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates. MMP12(arrow) was detected using the purified antibody.