Nanog (D2A3) XP ® Rabbit mAb (Mouse Specific)

• Supplier: Cell Signaling Technology
• Catalog: 8822
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D2A3
• Applications: WB IP ICC/IF FC ChIP
• Species Reactivities: Mouse
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein.
• Description: Rabbit Monoclonal
• Gene: Nanog
• Conjugate: Mouse Specific

Product images

Western blot analysis of extracts from F9 and murine iPS cells using Nanog (D2A3) XP ® Rabbit mAb (Mouse Specific).

Confocal immunofluorescent analysis of F9 (left) and NIH/3T3 (right) cells using Nanog (D2A3) XP ® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red).

Flow cytometric analysis of NIH/3T3 (blue) and F9 (green) cells using Nanog (D2A3) XP ® Rabbit mAb (Mouse Specific).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 F9 cells and either 5 μl of Nanog (D2A3) XP ® Rabbit mAb (Mouse Specific) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Mouse Oct-4 Promoter Primers #4653, SimpleChIP ® Mouse XIST Intron 1 Primers #4659, and SimpleChIP ® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.