Western blot analysis of extracts from NTERA2 and NCCIT cells using Nanog Antibody.
Confocal immunofluorescent analysis of NTERA2 cells (left) and HeLa cells (right) using Nanog Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Nanog Antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NCCIT cells and either 20 μl of Nanog Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human Oct-4 Promoter Primers #4641, SimpleChIP ® Human Sox2 Promoter Primers #4649, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.