Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated)
| Name | Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated) |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 8785 |
| Prices | $246.00 |
| Sizes | 100 µl (20 immunoprecipitations) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D1G10 |
| Applications | IP ChIP |
| Species Reactivities | Mouse |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein. |
| Description | Rabbit Monoclonal |
| Gene | Nanog |
| Conjugate | Mouse Specific; ChIP Formulated |
| Supplier Page | Shop |
Product images
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 F9 cells and either 5 μl of Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Mouse Oct-4 Promoter Primers #4653, SimpleChIP ® Mouse XIST Intron 1 Primers #4659, and SimpleChIP ® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.