Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-MLCK-long antibody in mouse brain tissue lysate. MLCK-long (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
MLCK Antibody (M1) western blot of SK-BR-3 cell line lysates (35 ug/lane). The MLCK antibody detected the MLCK protein (arrow).
Western blot of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.