MYLK

Gene Symbol MYLK
Entrez Gene 4638
Alt Symbol AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK
Species Human
Gene Type protein-coding
Description myosin light chain kinase
Other Description kinase-related protein|myosin light chain kinase, smooth muscle|myosin, light polypeptide kinase|smooth muscle myosin light chain kinase|telokin
Swissprots O95799 Q14844 Q17S15 O95798 B4DUE3 Q5MYA0 Q6P2N0 Q15746 O95796 Q9UBG5 O95797 Q16794 Q9UIT9 Q7Z4J0 D3DN97 Q9C0L5 Q3ZCP9 Q9UBY6 Q5MY99
Accessions AAD51380 AAD51381 EAW79435 EAW79436 EAW79437 EAW79438 EAW79439 EAW79440 EAW79441 EAW79442 EAW79443 EAW79444 EAW79445 EAW79446 Q15746 AB037663 BAB21504 AF069601 AAD15921 AF069602 AAD15922 AF069603 AAD15923 AF069604 AAD15924 AF096773 AAD54017 AF096774 AAD54018 AF096775 AAD54019 AF172084 AAQ13607 AK022278 AK025953 AK127437 AK300610 BAG62305 AK311053 AK314412 BAG37033 AK314443 BAG37052 AL832211 AY339601 AAQ02673 AY424269 AAR29061 AY424270 AAR29062 BC015838 BC017811 AAH17811 BC034911 BC040115 BC062755 BC064420 AAH64420 BC064695 BC100761 AAI00762 BC100762 AAI00763 BC100763 AAI00764 BC107783 AAI07784 BC113456 AAI13457 BC113458 AAI13459 BM723621 BU678390 CA391484 CA391924 CA432076 CR749364 CAH18217 DQ642691 ABG25912 DQ642692 ABG25913 U48959 AAC18423 X85337 CAA59685 X90870 CAA62378 Y09530 CAA70723 XM_011512860 XP_011511162 XM_011512861 XP_011511163 XM_011512862 XP_011511164 NM_053025 NP_444253 NM_053026 NP_444254 NM_053027 NP_444255 NM_053028 NP_444256 NM_053031 NP_444259 NM_053032 NP_444260 NM_005965 NM_053029 NM_053030
Function Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in
Subcellular Location Cytoplasm. Cell projection, lamellipodium. Cleavage furrow. Cytoplasm, cytoskeleton. Note=Localized to stress fibers during interphase and to the cleavage furrow during mitosis.
Tissue Specificity Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF). {ECO:0000269|PubMed:10536370, ECO:0000269|PubMed:16835238, ECO:0000269|PubMed:20053363, ECO:0000269|PubMed:8575746}.
Top Pathways Focal adhesion, Calcium signaling pathway, Vascular smooth muscle contraction, Gastric acid secretion, Oxytocin signaling pathway

MYLK (A-8) - sc-365352 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MYLK (A-8): sc-365352. Western blot analysis of MYLK expression in BC3H1 whole cell lysate.

MYLK (C-18) - sc-22226 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MYLK (C-18): sc-22226. Western blot analysis of MYLK expression in human colon tissue extract.

MYLK (H-195) - sc-25428 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MYLK (H-195): sc-25428. Immunoperoxidase staining of formalin fixed, paraffin-embedded human vulva/anal tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

MYLK (MLJ-16) - sc-69869 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

MYLK (MLJ-18) - sc-69870 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

MYLK (L-18) - sc-9452 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MYLK (L-18): sc-9452. Western blot analysis of MYLK expression in A-10 whole cell lysate.

MYLK (N-17) - sc-22223 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MYLK (N-17): sc-22223. Western blot analysis of MYLK expression in BC<sub>3</sub>H1 whole cell lysate.

MYLK (A-20) - sc-12450 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MYLK (A-20): sc-12450. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of smooth muscle cells.

MYLK (MLJ-9) - sc-69868 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

MYLK (MLJ-7) - sc-69876 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

MYLK (P-19) - sc-22224 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MLCK (P-19): sc-22224. Western blot analysis of MLCK expression in HISM whole cell lysate.

p-MYLK (Ser 439) - sc-16800 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-MYLK (Ser 439)-R: sc-16800-R. Western blot analysis of MYLK phosphorylation in untreated (A) and lambda protein phosphatase treated (B) MIA PaCa-2 whole cell lysates.

p-MYLK (Tyr 471) - sc-17183 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-MYLK (Tyr 471)-R: sc-17183-R. Western blot analysis of MYLK phosphorylation in untreated (A) and lambda protein phosphatase treated (B) MIA PaCa-2 whole cell lysates.

Anti-Myosin light chain kinase (phospho S1760) antibody - ab200809 from Abcam

Prices $359.00
Sizes 10 tests
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Myosin light chain kinase (phospho S1760) antibody (ab200809) at 1/1000 dilutionLane 1 : Extracts of COS cells transfected with muscle Myosin light chain kinase, unstimulatedLane 2 : Extracts of COS cells transfected with muscle Myosin light chain kinase phosphorylated in vitro by 0.05 µg of PKALane 3 : Extracts of COS cells transfected with muscle Myosin light chain kinase phosphorylated in vitro by 0.05 µg of PKA with the non-phosphopeptide corresponding to the phosphopeptide immunogenLane 4 : Extracts of COS cells transfected with muscle Myosin light chain kinase phosphorylated in vitro by 0.05 µg of PKA with a generic phosphoserine-containing peptideLane 5 : Extracts of COS cells transfected with muscle Myosin light chain kinase phosphorylated in vitro by 0.05 µg of PKA with the phosphopeptide immunogenLane 6 : Extracts of COS cells transfected with muscle Myosin light chain kinase phosphorylated in vitro by 0.05 µg of PKASecondarygoat F(ab’)2 anti-rabbit IgG HRP

Anti-Myosin light chain kinase antibody - ab130135 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Myosin light chain kinase antibody (ab130135) at 0.03 µg/ml (incubated for 1 hour) + Human Colon lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-Myosin light chain kinase antibody - ab203292 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of Formalin-fixed, paraffin embedded Rat colon tissue labeling Myosin light chain kinase with ab203292 at 1/200 dilution, followed by secondary detection and DAB staining.

Anti-Myosin light chain kinase antibody - ab55475 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab55475 Myosin light chain kinase antibody at 1ug/ml.

Anti-Myosin light chain kinase antibody [EP1458Y] - ab76092 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1458Y
Immunohistochemical analysis of paraffin embedded human smooth muscle, using ab76092, at 1/100 dilution.

Anti-MYLK Antibody (phospho-Ser1760) - LS-C25729 from LifeSpan Bioscience

Prices $495.00
Sizes 10 blot
Host Rabbit
Clonality Polyclonal

Anti-MYLK Antibody (aa908-938, APC) - LS-C279870 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MYLK Antibody (aa908-938, AP) - LS-C279869 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MYLK Antibody (aa908-938, FITC) - LS-C279872 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MYLK Antibody (aa908-938, Biotin) - LS-C279871 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MYLK Antibody (aa908-938, Azide-free, HRP) - LS-C279873 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MYLK Antibody (aa1691-1740) - LS-C200246 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HT-29 cells using MYLK antibody.
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