Detection of Human Mineralocorticoid R/NR3C2 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 ug/ml of Human Mineralocorticoid R/NR3C2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 110 kD (as indicated). This experiment was conducted under reducing conditions.