Anti-NANOG Antibody (phospho-Ser71)
| Name | Anti-NANOG Antibody (phospho-Ser71) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C163646 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Monkey |
| Purity/Format | Protein A purified |
| Blocking Peptide | NAMPT / Visfatin Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | NANOG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent confocal image of SY5Y cells stained Phospho-Nanog-S71 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated Phospho-Nanog-S71 primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Phospho-Nanog immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
Dot blot of anti-Phospho-Nanog-S71 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.