Detection of Human NAT10 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human NAT10 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.