Anti-NCL / Nucleolin Antibody (aa428-455)
| Name | Anti-NCL / Nucleolin Antibody (aa428-455) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C164776 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC ICC/IF WB FC |
| Species Reactivities | Human |
| Purity/Format | Protein A purified |
| Blocking Peptide | NCKIPSD / AF3P21 Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | NCL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
NCL Antibody (Center E443) immunohistochemistry of formalin-fixed and paraffin-embedded human breast tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Fluorescent confocal image of HeLa cell stained with NCL Antibody (Center E443). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.
NCL Antibody (Center E443) western blot of HepG2 cell line lysates (35 ug/lane). The NCL antibody detected the NCL protein (arrow).
Western blot of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.