IHC of paraffin-embedded endometrium tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of kidney tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
Immunoprecipitation(IP) of NEK6 by using monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-NEK6 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.