Anti-Neu2 Antibody - Western Blot. Western blot of Affinity Purified anti-Neu2 antibody to detect Neu-2 present in a lysate expressing human Neu2 (1.0 ul loaded). Molecular weight marker (not shown) indicates a band of the expected MW (43 kD). The reactive lower molecular weight band is believed to represent a truncated form of this protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:1000. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-Neu2 Antibody - Western Blot. Western blot of Affinity Purified anti-Neu2 antibody to detect recombinant His tagged Neu-2 (1.0 ug loaded). Molecular weight marker (not shown) indicates a single band of the expected MW (43 kD). The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:1000. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.