Detection of Human Nexillin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Nexillin antibody used for IP at 6 ug/mg lysate. Nexillin was also immunoprecipitated by rabbit anti-Nexillin antibody BL13584, which recognizes a downstream epitope. For blotting immunoprecipitated Nexillin, rabbit anti-Nexillin antibody BL13585 was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.