Detection of Human Neogenin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody used for IP at 6 ug per reaction. Neogenin was also immunoprecipitated by rabbit anti-Neogenin antibody BL16480. For blotting immunoprecipitated Neogenin, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human and Mouse Neogenin by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Neogenin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.