Western blot analysis of caveolin-1 phosphorylation in untreated (A,D), pervanadate treated (B,E) and pervanadate and lambda protein phosphatase treated (C,F) A-431 whole cell lysates. Antibodies tested include p-caveolin-1 (Tyr 14)-R: sc-14037-R (A,B,C) and caveolin-1 (N-20): sc-894 (D,E,F).
Western blot analysis of caveolin-1 phosphorylation in untreated (A,D), pervanadate treated (B,E) and pervanadate and lambda protein phosphatase treated (C,F) A-431 whole cell lysates. Antibodies tested include p-caveolin-1 (Tyr 14)-R: sc-14037-R (A,B,C) and caveolin-1 (N-20): sc-894 (D,E,F).
p-caveolin-1 (Tyr 14)-R: sc-14037-R. Immunofluorescence staining of methanol-fixed A-431 cells treated with Pervanadate (Sodium Orthovanadate: sc-3540 + Hydrogen Peroxide: sc-20336) (A) untreated (B) and treated with Pervanadate and Lambda Phosphatase: sc-200312 (C).
Western blot analysis of caveolin-1 phosphorylation in untreated (A,D), pervanadate treated (B,E) and pervanadate and lambda protein phosphatase (sc-200312A) treated (C,F) ECV304 whole cell lysates. Antibodies tested include p-caveolin-1 (Tyr 14)-R: sc-14037-R (A,B,C) and caveolin-1 (N-20): sc-894 (D,E,F).